cDNA constructs

Plasmids encoding human K_v7.1, K_v7.2, K_v7.3, K_v7.4 and K_v7.5 (GenBank accession numbers: NM000218, {"type":"entrez-nucleotide","attrs":{"text":"AF110020","term_id":"4324686","term_text":"AF110020"}}AF110020, {"type":"entrez-nucleotide","attrs":{"text":"AF091247","term_id":"3929230","term_text":"AF091247"}}AF091247, {"type":"entrez-nucleotide","attrs":{"text":"AF105202","term_id":"4262522","term_text":"AF105202"}}AF105202 and {"type":"entrez-nucleotide","attrs":{"text":"AF249278","term_id":"9651966","term_text":"AF249278"}}AF249278 respectively) were kindly provided by Diomedes E. Logothetis (Virginia Commonwealth University, Richmond, Virginia, USA) and subcloned into pcDNA3. The K_v7.4(G302T/A304T), K_v7.4(L306I), K_v7.4(I308V) and K_v7.4(L306I/I308V) mutants were produced by Pfu DNA polymerase with a QuickChange kit (Stratagene, USA). The mutants' identity was confirmed with DNA sequencing.