4.2. RNA Isolation

YC Yueh-Hua Chung
SL Sung-Chou Li
YK Ying-Hsien Kao
HL Hao-Lun Luo
YC Yuan-Tso Cheng
PL Pey-Ru Lin
MT Ming-Hong Tai
PC Po-Hui Chiang
request Request a Protocol
ask Ask a question
Favorite

Total RNA was extracted by Trizol® Reagent (Invitrogen, Waltham, MA, USA) according to the instruction manual. The quality of isolated RNA was analyzed by using an RNA 6000 Lab-chip kit (Agilent Technologies, Santa Clara, CA, USA) on a Bioanalyzer 2100 (Agilent Technology, Santa Clara, CA, USA) and quantified at OD 260 nm by using an ND-1000 spectrophotometer (Nanodrop Technology, City, State, USA).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A