Total RNA was extracted by Trizol® Reagent (Invitrogen, Waltham, MA, USA) according to the instruction manual. The quality of isolated RNA was analyzed by using an RNA 6000 Lab-chip kit (Agilent Technologies, Santa Clara, CA, USA) on a Bioanalyzer 2100 (Agilent Technology, Santa Clara, CA, USA) and quantified at OD 260 nm by using an ND-1000 spectrophotometer (Nanodrop Technology, City, State, USA).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.