cDNA plasmids and siRNAs

The wild-type sLZIP, N-terminal sLZIP (1–220), C-terminal sLZIP (221–354) and CC-terminal sLZIP (296–354) constructs were generated by a PCR [14]. All point mutations in two LxxLL motifs of sLZIP were generated by a QuikChange site-directed mutagenesis kit (Stratagene, Santa Clara, CA, USA). The 21-nucleotide sequences for the siRNA targeting sLZIP were 5′-CGUCGUUGAACAUUCUCAGdTdT-3′ (sense) and 5′-CUGAGAAUGUUCAACGACGdTdT-3′ (antisense). All siRNAs were synthesized from Bioneer (Daejeon, South Korea).