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Multiplexed bead-based cytokine immunoassays.
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CD34+ HPCs were mock infected or HCMV infected for 4 h before treatment with AG1478 (EGFRK inhibitor) or DMSO. After 24 h at 37°C and 5% CO2, the supernatants were collected and frozen at −20°C prior to analysis. Undiluted supernatants were analyzed to quantify the protein levels of TGF-β using a Bio-Plex Pro TGF-β immunoassay (Bio-Rad Laboratories, Hercules, CA) and a panel of 40 human chemokines using a Bio-Plex Pro human chemokine immunoassay (Bio-Rad) according to the manufacturer's protocol.
Copyright and License information: ©2017 American Society for Microbiology.
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