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6-OHDA-induced PD rat model
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Rats weighing 240–300 g were anesthetized with intraperitoneally (i.p.) administered pentobarbital sodium (60 mg/kg). The 6-OHDA (4 μg 6-OHDA dissolved in 4 μL sterile saline containing 0.02% ascorbic acid) was unilaterally injected into the right hemisphere (coordinates from bregma: AP, -4 mm; ML, +1.65 mm; DV, -8 mm) according to the Paxinos and Watson's rat brain atlas (1998) with a Hamilton syringe (0.46 mm in diameter, blunt tip) at a rate of 0.5 μL per minute. The needle was kept in place for 8 min and then slowly withdrawn over a 3 to 4 min period.
Copyright and License information: ©2017 Wang et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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