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Immunoprecipitation of hydroxymethylated DNA was performed with a mouse monoclonal antibody against 5-hydroxymethylcytosine (5hmC) using the hMeDIP kit (Diagenode, Cat. no. C02010031) following the manufacturer's instructions. DNA samples were sheared at 4°C using a Diagenode Bioruptor. DNA (1 μg) was used for each IP; recovered DNA was analyzed by real-time PCR (Table S3). Hydroxymethylated, methylated, and unmethylated DNA controls and primers for their amplification were provided with the kit.
Copyright and License information: ©2017 The Author(s). Published with license by Taylor & Francis Group, LLC
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
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