Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Small interfering RNA (siRNA) transfection
Ask a
question
Favorite
Glut1 specific siRNA (catalogue no. sc-35,493; Santa Cruz Biotechnology, Inc.) was transfected into MCF-7 cells by Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. Scrambled siRNA (catalogue no. sc-37,007; Santa Cruz Biotechnology, Inc.) was used as a negative control. Reduced serum medium was changed into complete medium 6 h subsequent to transfection. Cells were harvested 48 h subsequent to transfection, and then used for additional experiments.
Copyright and License information: ©2017 Sun et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
