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Spectrophotometric determination of carotenoid and chlorophyll content
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Twenty mL of acetone–hexane (4:6, v/v) were added to 100 mg of lyophilised plant material and thoroughly mixed. Upon separation of the two phases, the absorbance was determined in the supernatant at 453, 505, 645 and 663 nm. Based on Nagata and Yamashita equations, total carotenoids, chlorophyll-a and chlorophyll-b contents were determined [18].
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