Immunohistochemistry to measure BDNF and TrkB expression

YT Yi-Fang Tsai
LT Ling-Ming Tseng
CH Chih-Yi Hsu
MY Muh-Hwa Yang
JC Jen-Hwey Chiu
YS Yi-Ming Shyr
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The protein expression levels of BDNF and TrkB in a tissue array containing 95 tumor samples from the archives of the Department of Pathology were measured after immunohistochemical staining for BDNF (Genetex, GTX62495,CA, USA) and TrkB (BD, 610101); the slides were evaluated by an expert in pathology. The degree of positiveness for protein expression was measured in a semi-quantified manner and is expressed as (0), <10%, (1), 11–25%, (2), 26–50%, (3) >50% of the tumor cells examined.

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