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Cell lines were seeded in 96‐well plates at the cells/well density of 5.0 × 103 (for HCT116, MCF‐7, and SJSA‐1) and 1.0 × 104 (for MCF10A and HMVEC‐D). Cells were thereafter treated with serial dilutions (1.85–75 μm) of DIMP53‐1 for 24 and 48 h, and its effect on cell proliferation was analyzed by sulforhodamine B (SRB) assay (Soares et al., 2015a) with the determination of IC50 (concentration that causes 50% of growth inhibition) values. The solvent (DMSO 0.25%) was included as control.
Copyright and License information: ©2017 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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