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Approximately 107 cells were resuspended in 500 μL of PBS with Roche Complete protease inhibitor tab and subjected to 3 freeze/thaw cycles. Whole cell lysates were aliquoted into PCR strips and exposed to a thermal gradient ranging from 44.5°C to 67°C. Tubes were spun down at 20,000 g for 20 min, and supernatants were collected followed by separation by SDS-PAGE.
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