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Lipopolysaccharide (LPS) Lung Inflammation Model
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BALB/c mice (6–7 wk old) were anesthetized by isoflurane inhalation. LPS (from E. coli serotype 0127:B8; Sigma) (50 μg) in 20 μL of saline (0.9% NaCl) was administered via intranasal instillation. The control group consisted of mice without any treatment because simple administration of vehicle (saline) is enough to induce lung inflammation. Twenty-four hours after LPS administration, the mice were sacrificed for biodistribution studies and the lungs were saved for histopathologic analysis.
Copyright and License information: ©2017 by the Society of Nuclear Medicine and Molecular Imaging.
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