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Bacterial growth in THB at 37°C in ambient air was monitored using an OD monitor (ODBox-C; TAITEC, Koshigaya, Japan). Overnight culture (60 μL) was diluted in 6 mL of fresh THB. The cultures were shaken at 160 rpm and the optical density (600 nm) was measured every 5 min for 24 h. The experiment was performed five times.
Copyright and License information: ©2017 Banno et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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