Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
SKOV3 cells were seeded into a 96-well plate at a density of 5×104 cells/well and transfected as described above. At 48 h following transfection, 10 µl MTT solution (5 mg/ml, Sigma-Aldrich; Merck KGaA) was added to each well and incubated at 37°C for 4 h. Then, 100 µl solubilization solution [50% dimethylsulfoxide and 20% SDS (pH 4.8)] was added to each well followed by agitation for 10 min. The absorption values were measured at 570 nm.
Copyright and License information: ©2017 Ye et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
