Prediction Formula

Babak Basiri; Mandi M. Murph; Michael G. Bartlett

Improve Research Reproducibility A Bio-protocol resource

Home
Protocols

Concise Method

Prediction Formula

BB Babak Basiri

MM Mandi M. Murph

MB Michael G. Bartlett

This method is extracted from research article: J Am Soc Mass Spectrom, Apr 2017

Assessing the interplay between the physicochemical parameters of ion-pairing reagents and the analyte sequence on the electrospray desorption process for oligonucleotides

DOI: 10.1007/s13361-017-1671-6

Ask a question

Favorite

The signal intensity prediction formula is as follows. We have also supplemented a Microsoft Excel sheet containing this formula with the online version of this manuscript for the convenience of our readers. Please note that Content A, T, C and G need to be entered as decimals.

Predicted Normalized Signal Intensity = −0.00656 MW − 5.43532 Density + 0.02322 Boiling Point + 1.61079 pKa − 0.12832 Proton Affinity − 0.14625 Gas Phase Basicity + 0.23521 Partition Coefficient + 0.00005 Water Solubility − 0.00012 Vapor Pressure + 0.00340 Henry’s Law Constant + 4.75149 Content A + 7.00368 Content T + 4.39043 Content C + 0.55245 Content G + 47.72180.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

Post a Question

0 Q&A

Share your protocol with your peers.

Submit a Preprint Protocol