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Sequence data were processed and demultiplexed in QIIME (Caporaso et al., 2010) using the default quality control parameters. Sequences were binned into OTUs with a de novo picking strategy using UCLUST (Edgar, 2010) at a minimum sequence identity of 97%. Chimeras were removed with ChimeraSlayer (Haas et al., 2011) along with sequences identified as chloroplasts or mitochondria.
Copyright and License information: ©2017 International Society for Microbial Ecology
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