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In vitro MMP gelatinase assay (In situ zymography)
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In situ detection of MMP9 activity was carried out by overlaying cells grown in an 8-well chamber slide with 100 μg/ml quenched fluorescein-labeled gelatinase substrate, DQ-gelatin (Molecular Probes, Eugene OR) for 2 hours at 37 °C. Slides were washed and fixed with 4% paraformaldehyde, and imaged using a flourescence microscope (EVOS, ThermoFisher Scientific, Walthem, MA).
Copyright and License information: ©2016
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