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Proteins were separated by SDS‐PAGE and transferred to nitrocellulose membranes (Bio‐Rad, Hercules, CA, USA), after which the membranes were blocked in PBS/Tween‐20 containing 5% non‐fat milk. Then the membranes were probed with primary antibodies against eIF3c (Santa Cruz Biotechnology, Santa Cruz, CA, USA) overnight at 4°C. Immune‐reactive bands were detected using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, Waltham, MA, USA).
Copyright and License information: ©2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
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