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Histological analysis and IHC staining of ovary
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Ovaries were fixed in 4% paraformaldehyde (pH 7.5) overnight at 4 °C, dehydrated in 50%, 70%, in a graded ethanol series, cleared in xylene three times and finally embedded in paraffin wax. The treated ovaries were sectioned consecutively at 8 μm for hematoxylin and eosin (H&E) staining and IHC staining. Serial ovarian sections were photographed and follicles were counted in every fifth sections using the Image J software and cell counter plugin.
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Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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