ICR male mice (18–22 g) were obtained from Yangzhou University (Yangzhou, China). Animals were housed with a 12-h light–dark cycle at 22 °C and relative humidity of 55 ± 5%, and had free access to food and water. All experiments protocols involving animals were carried out according to guidelines set by Institutional Animal Care and Use Committee of China Pharmaceutical University (Jiangsu, China).
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