S. pombe genetics

Standard media and methods of propagation were used throughout (Moreno, et al. 1991). All S. pombe strains were constructed by tetrad analysis, and double mutants were selected as non-parental ditypes. For each genotype, multiple progeny were tested and backcrossed to exclude the presence of suppressor mutations occurring in the genome.

For chronic growth assays, cells were streaked on agar plates containing supplemented yeast extract plus glucose (YES) containing phloxin B. The plates were incubated at 25°C for 5 days, or 30°C for 4 days.