Toxicity evaluation

The extract of AM was evaluated for toxicity in rats. To determine acute toxicity, single oral administration of AM extract at different doses (500, 1000, and 2000 mg/kg body weight) was done to different groups of Wistar rats (n = 6) of either sex. Mortality and general behavior of the animals were observed continuously for the initial 4 h and intermittently for the next 6 h and then again at 24, 48 and 72 h following extract administration. To determine chronic toxicity autopsy was performed after 42 days of extract treatment to check any abnormality in the liver, kidney, gastrointestinal tract, spleen and heart. Serum creatinine and urea, ALT and AST were also evaluated to check the toxicity to kidney and liver, respectively. Eugenol (EG) was evaluated for its cytotoxicity in pancreatic MIN6 cells, and no toxic effects were observed (IC₅₀ > 200 μM).