2.1. Reagents and antibodies

Primary antibodies used in this study include the following: Rabbit polyclonal anti‐MCT1 (sc‐50324, Santa Cruz, Dallas, TX, USA); mouse polyclonal anti‐MCT1 (ab90582, Abcam, Cambridge, MA, USA); goat polyclonal anti‐MBP (sc‐13914, Santa Cruz); mouse monoclonal anti‐NG2 and rabbit polyclonal anti‐NG2 (ab129051, Abcam); rabbit polyclonal anti‐PDGFRα+ (#3164, Cell Signaling, Beverly, MA, USA). MCT1‐transfected 293T cells lysate, mouse heart extract, and rat skeletal muscle extract were used as positive controls, and MCT1 siRNA (sc‐40115, Santa Cruz) was used as knockdown control for the MCT1 antibodies. The secondary antibodies used for immunofluorescence were Alexa Fluor 488, or 594 donkey anti‐mouse, rabbit, or goat IgG (Thermo Fisher scientific, Eugene, OR, USA), and the HRP‐conjugated secondary antibodies used for Western blot analysis were from Thermo Fisher Scientific. For antibody validation, MCT1 knockdown was carried out using Lipofectamine RNAiMAX (Thermo Fisher Scientific) on cells seeded 24 hours prior.