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Autophagy detection by Lysotracker
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Lysotracker DND-26 (#8783, Cell Signaling Technology, Inc., Danvers, MA, USA) was diluted 1:10000 directly into cell culture media to make 100 nM final concentration. Live cells were stained with LysoTracker Green DND-26 and Hoechst 34580 for lysosomes and nucleus, respectively, and were analyzed using fluorescent microscopy immediately after treatment without fixation.
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