Steroid hormone and gonadotropin quantification

LM Leticia Morales-Ledesma
JR Juan Antonio Díaz Ramos
AH Angélica Trujillo Hernández
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E2 and P4 serum concentrations were quantified by solid phase RIA using commercial Coat-A-Count reagents (Diagnostic Products Corp., Los Angeles, CA, USA). The LH and FSH levels in the serum (ng/ml) were measured using the double antibody RIA technique, with reagents and protocols kindly supplied by the NIADDK National Pituitary Program (Bethesda, MD, USA).

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