E2 and P4 serum concentrations were quantified by solid phase RIA using commercial Coat-A-Count reagents (Diagnostic Products Corp., Los Angeles, CA, USA). The LH and FSH levels in the serum (ng/ml) were measured using the double antibody RIA technique, with reagents and protocols kindly supplied by the NIADDK National Pituitary Program (Bethesda, MD, USA).
Copyright and License information: The Author(s). ©2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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