3.13. Statistical Analysis

All experiments were run in triplicate. The results represented are means ± standard deviation (± SD, n = 3). Statistical analyses were done by using Origin 7.0 SRO software package (OriginLab Corporation, Northampton, MA, USA, 1991–2002) and Microsoft Office Excel 2010 software. Significant differences were calculated by ANOVA (p < 0.05). For optimization experiments, the models quality was determined by analysis of variance (ANOVA) of regression. Calculations and graphs, single and multi-response optimization was conducted using Design-Expert^® Version 7.0.0, Stat-Ease, Inc., Minneapolis, MN, USA (2005).