Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
CD4+T cells were enriched from SPN and LN by complement lysis of cells bearing CD8 (3.155), MHC class II (M5/114), and heat-stable Ag (J11D) as described(38) (ATCC). CD25+ and CD25− cells were enriched using MACS column (Miltenyi Biotech) and sorted (FACS Aria, BD) for KJ126+ Foxp3-GFP+ CD62L+ CD44− naïve Tregs and KJ126+ Foxp3GFP− CD62L+ CD44− naïve Tconv cells.
Copyright and License information: ©2016
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
