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Micro-CT analysis

LW Liang Wang
PJ Peng Jia
YS Yu Shan
YH Yanming Hao
XW Xiao Wang
YJ Yu Jiang
YY Ye Yuan
QD Qiaoqiao Du
HZ Hui Zhang
FY Fan Yang
WZ Wen Zhang
MS Mao Sheng
YX Youjia Xu
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Mouse femora and lumbar vertebrae were dissected at several points in time, and any attached soft tissue was completely removed. Collected bone specimens were then fixed in 4% paraformaldehyde (PFA) and analyzed by micro-CT (SkyScan 1176; Kontich, Belgium). The scanners were set at a voltage of 50 kV, a current of 500 µA, and a resolution of 9 µm. Image software (NRecon v1.6) and data analysis software (CTAn v1.13.11.0) were used for three-dimensional (3D) reconstruction of the trabecular bone.

After scanning, regions of interest (ROI) were defined by analysis software and limited to the trabecular regions. 3D trabecular bone images were reconstructed based on the ROI. Mouse femoral ROI were drawn starting from 540 µm proximally to the distal growth plate over 1.35 mm toward the diaphysis. Lumbar vertebra ROI was drawn in the middle trabecular area with 2 mm of thickness. Trabecular bone parameters were calculated, including BMD (g/cm3), BV/TV (%), Tb.Th (mm), Tb.N (per mm) and Tb.Sp (mm).

Copyright and License information:  ©2017 Wang et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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