Oral glucose tolerance test in diabetic mice

Oral glucose tolerance test (OGTT) was performed after 4 weeks administration. After overnight fasting, the mice were weighed, and ‘0’ minute blood glucose was taken by glucose meters (Sinocare Inc., China) from control and experimental mice. Without delay, a glucose solution (2.5 g/kg body weight) was administered by oral gavage. Blood glucose were taken at 30, 60, 90, 120, and 240 min after glucose administration using glucose meters [23]. Calculation of the area under the curve was made according to Eq (I) [24].