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Platelet rich plasma (PRP) samples stored at 30°C were preheated to 37°C for 2 minutes and stirred at 1000 rpm in an aggregometer (Chronolog Model 700, Labmedics, Manchester, United Kingdom) before adding adenosine 5′-diphosphate (ADP; 10 µM). The change in optical density relative to platelet poor plasma (100% aggregation reference) was recorded for 6 minutes, and maximum aggregation extent was used for data analysis.
Copyright and License information: ©2016 by The American Society of Hematology
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