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Paraffin sections were dewaxed and hepatic cell apoptosis was detected by terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) assay using the TUNEL assay kit (Roche, Shanghai, China) according to the manufacturer’s protocol. Nuclei were labeled by DAPI staining (Biohao Biotech, Shanghai, China). Sections were then observed under a confocal laser microscope (Olympus, Japan). The percentage of apoptotic cells was calculated by counting TUNEL-positive cells in five randomly selected fields on each slide.
Copyright and License information: ©2017 The Author(s).
This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
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