Adoptive transfer of CD8 T cells

RS Robert K. Strother
DD Derek B. Danahy
DK Dmitri I. Kotov
TK Tamara A. Kucaba
ZZ Zeb R. Zacharias
TG Thomas S. Griffith
KL Kevin L. Legge
VB Vladimir P. Badovinac
request Request a Protocol
ask Ask a question
Favorite

To generate a primary CD8 T cell response, 103 naïve Thy1.1/Thy1.1 OT-1 CD8 T cells or Thy1.1/Thy1.1 P14 CD8 T cells were obtained from the peripheral blood of young naïve TCR-Tg mice and transferred into Thy1.2 recipients 1 d before infection with 103 LM-OVA or 106 attLM-gp33, respectively.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A