To generate a primary CD8 T cell response, 103 naïve Thy1.1/Thy1.1 OT-1 CD8 T cells or Thy1.1/Thy1.1 P14 CD8 T cells were obtained from the peripheral blood of young naïve TCR-Tg mice and transferred into Thy1.2 recipients 1 d before infection with 103 LM-OVA or 106 attLM-gp33, respectively.
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