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Electrophoretic mobility shift assay (EMSA)

DS Davis M. Seelig
DI Daisuke Ito
CF Colleen L. Forster
UY Una A. Yoon
MB Matthew Breen
LB Linda J. Burns
VB Veronika Bachanova
KL Kerstin Lindblad-Toh
TO Timothy D. O’Brien
SS Stephen C. Schmechel
AR Anthony E. Rizzardi
JM Jaime F. Modiano
ML Michael A. Linden
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Nuclear extracts of lymphoma cells were prepared as described above. DNA-binding activity of NFκB was analyzed by EMSA using a 5 μg protein extract and a palindromic NFκB binding sequence probe (LI-COR). EMSA supershift assay was done by using 1–2 μg of the same antibodies described for the immunoblotting studies above. Detection was performed using the Odyssey Infrared Imaging System (LI-COR).

Copyright and License information:  ©2016

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