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To generate PLX8394-resistant lines, HCC364 cells were exposed to an increasing concentration of PLX8394 from 500 nM to 10 μM. Individual populations of cells were confirmed to be drug resistant by standard cell viability assays. The genetic identity of each subclone with that of the parental cells was verified by short-tandem repeat analysis conducted at Johns Hopkins University according to established protocols.
Copyright and License information: ©2016
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