2.6. Dynamic light scattering measurement (DLS)

Liposome and proteoliposome size distributions were determined by dynamic light scattering (DLS) using a N5 Submicron Particle Size Analyzer (Beckman Coulter, Inc., Fullerton, CA) with a 25 mW HeNe laser with fixed scattering angle of 90° as light source. Samples (prepared under the same conditions as described in Sections 2.4 and 2.5) were filtered through 0.8 μm-pore size Millipore® membranes five times before DLS measurements. The average liposome diameters were measured at 25 °C by taking the unimodal distribution [1,24].