3.4. Novozym 435-Catalyzed Esterification of Methyl Glucoside and Fatty Acids

Novozym 435 (normally 10 mg) was added to a 5-mL capped test tube containing methyl glucoside (0.058 g, corresponding to 0.3 mol/L of the reaction system, only partially dissolved), a fatty acid (0.3 M), and molecular sieves (150 mg) in 1 mL of solvent (pure 2M2B or IL or IL/2M2B mixture), which was agitated at 300 rpm and 45 °C. Periodically, a 10-µL sample was taken and 3 times diluted with DMSO for HPLC analysis as indicated below. The conversion was calculated as in Section 3.3, based on the total amount of methyl glucoside added to the reaction system.