Mouse airway exposure and challenge model

On days 0 and 7, naïve mice were lightly anesthetized with tribromoethanol or isoflurane and administered 100 μg OVA intranasally (i.n.) in the presence or absence of 100 ng or serial dilutions of IL-33 or IL-1β in 50 μl of endotoxin-free PBS. On day 11, the mice were euthanized by an overdose of pentobarbital, and mediastinal lymph nodes (mLNs) were collected. In some experiments, mice were exposed i.n. to Alternaria (50 μg), cockroach (50 μg), or HDM (400 μg) extracts, or bromelain (10 μg), or to Alternaria (50 μg) plus OVA (10 μg). Some mice were then challenged i.n. with 100 μg OVA using the protocols described in the figures and figure legends.