Development of the Bd21 X Bd1-1 Recombinant Inbred Line Population

A Brachypodium distachyon RIL population was generated from a cross between inbred lines Bd21 (female) and Bd1-1 (male). A single F1 plant was self-pollinated and the resulting F2 seeds were propagated by single-seed descent to the F₆ generation. Individual F₆ plants were then selfed to produce 142 F_6:7 RILs for use in genetic analysis and gene mapping (Fig. 3A). Several F_7:8 plants per line were planted to bulk F8 seed.