2.7. Three‐dimensional reconstructions

Three‐dimensional reconstructions of neuropils were performed manually with Amira 5.33 based on anti‐synapsin labeling and in some preparations background staining. Characteristic vertices of the neuropils were marked on different levels of the image stacks for subsequent computation of the structures. Polygonal surfaces were created with the module SurfaceGen. Neuronal reconstructions were done with the SkeletonTree tool within Amira 5.33 (Evers, Schmitt, Sibila, & Duch, 2005; Schmitt, Evers, Duch, Scholz, & Obermayer, 2004).