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Cells and cell culture conditions

MK Mine Koruyucu
CA Cansu Akay
SS Seyhun Solakoglu
KG Koray Gencay
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For this study, commercially isolated and registered human dental pulp stem cells (Lonza Group, Switzerland) were kept in a nitrogen tank (-196 oC) until the beginning of the cell transplantation process, and on the day of the experiment, they were removed from the nitrogen tank and dissolved in a water bath and brought to room temperature. The medium for the cultivation and reproduction of cells was prepared as recommended by the manufacturer. The medium from the flasks was removed in the process of passaging. The flasks were washed with 2 ml trypsin EDTA (Sigma- Aldrich T4049, St. Louis, MO, USA) was added to the flasks. After 2 h of incubation, the cells were separated from the flask base. 4 ml TNS (Trypsin neutralization solution) was used to wash the flasks and the cells were seeded into new sterile falcon tubes. The tubes were centrifuged at 1500 rpm for 5 min and cultivated in a new culture flask. Cells at the 3rd passage were used in assays.

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