The release of LDH from cervical cancer cells was assessed using the LDH assay kit (Dojindo, CK12). SiHa cells, at a density of 8 × 10^3 per well, were seeded into a 96-well microplate and cultured for 48 h in medium supplemented with DOX. According to the manufacturer’s protocol, 100 µl of working solution was added to each well. After 30 min of incubation, stop solution was added, followed by detection using a microplate reader (BioTeck, Synergy H1) at 490 nm, and the % of LDH released was then calculated applying the formula: 100 × (experimental LDH release-culture medium background)/(maximum LDH release- culture medium background). The experiment was performed three independent times.
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