Mecp2-e1 mouse breeding and phenotyping

OS Osman Sharifi
VH Viktoria Haghani
KN Kari E Neier
KF Keith J Fraga
IK Ian Korf
SH Sophia M Hakam
GQ Gerald Quon
NJ Nelson Johansen
DY Dag H Yasui
JL Janine M LaSalle
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Mecp2e1-/+ female dams were bred with C57BL6/J wild-type male mice (Jackson Labs strain 000664) to produce Mecp2e1-/+ Mecp2e1+/+, Mecp2-/y and Mecp2+/y littermates for single-nuclei RNA seq. Mice were weaned at 21 days post-natal and genotyped using DNA from tail snips as described4. Mouse disease scoring was performed blinded based on a seven-point scale with points assigned for matted fur, unusually large abdomen, skin lesions, hypoactivity and responses to tail suspension4. All animal procedures were approved by the UC Davis Institutional Animal Use and Care Committee (IACUC). We have complied with all relevant ethical regulations for animal use.

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