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Anthocyanin extraction and HPLC analysis
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The procedure for anthocyanin extraction was conducted following a previously published method [24]. High-performance liquid chromatography (HPLC) analysis was performed with a Waters Alliance 2695 system (Waters Corp., Milford, MA, USA), utilizing a reverse-phase C18 column (4.6 × 250 mm, 5 μm; YMC Co., Ltd., Kyoto, Japan). The absorption at 520 nm was recorded and the quantification of anthocyanin was accomplished by comparing with authentic standard C3G.
Copyright and License information: ©2024 The Author(s) 2024. Published by Oxford University Press on behalf of Nanjing Agricultural University.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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