Mouse Xenograft Model.

Female NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ (NGS) mice were obtained from The Jackson Laboratory. All animal experimental procedures received approval from the Animal Care and Use Committee of the Dana Farber Cancer Institute. Mice were fed a normal chow diet (-DOX) or, where indicated, a Doxycycline 625PPM 5AW9 Purple diet (TestDiet; 1816024-203). 5 × 10⁶ tumor bioluminescent cells were injected subcutaneously into the one flank per mouse. Tumor growth was assessed weekly using BLI over a 6-wk period. Upon reaching the study endpoint, mice were killed using CO₂. Subsequently, tumors were collected, and their dimensions were measured using the following formula: Width × Width/(height/2).