Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Cell viability was assessed using an MTT assay one day following treatment. For the assay, MTT reagent was diluted 10-fold into complete medium and incubated with cells in 96 well plates for 2 h. Absorbance was then analyzed at 490 nm using a multi-plate reader (Synergy 2, Biotek), blank-subtracted and normalized to the untreated cell values to give relative cell viability.
Copyright and License information: ©2017
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
