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Using a web server, users can access the highly sensitive miRNA prediction tool in plants known as the psRNATarget algorithm. The target viral mRNA region and host miRNAs are reversely complementary in the psRNATarget algorithm, which can be accessed at http://plantgrn.noble.org/psRNATarget/ (Dai and Zhao, 2011). Using the psRNATarget methodology, target-site accessibility is assessed by computing the unpaired energy (UPE). Using user-specified parameters and an expected cut-off value of 7, the interaction of miRNA and mRNA was calculated ( Table 1 ).
Copyright and License information: Copyright ©2024 Pandey, Srivastava, Ali, Gupta, Shahid and Gaur
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