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Growth curve and cell viability assay

SS Sameer Bikram Shah
YL Youhang Li
SL Shibo Li
QH Qing Hu
TW Tong Wu
YS Yanmeng Shi
TN Tran Nguyen
II Isaac Ive
LS Linda Shi
HW Hailong Wang
XW Xiaohua Wu
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Cell proliferation was determined by growth curves113. Briefly, U2OS and RPE1 cells as well as their derived cell lines were seeded at a density of 1 × 104 cells in 6 well cell culture dishes. Cell proliferation was assessed by counting trypsinized cells using Countess™ II FL automated cell counter (Thermo Fisher) every 24 hours. The cell number was normalized to that on day 1.

For the cell viability assay, cells were seeded in 96-well plates at a density of 2000 cells per well and treated with indicated concentrations of Olaparib for 72 hours. Subsequently, 100 μl of cell medium from each well was mixed with 20 μl Cell Counting Kit-8 (CCK-8, Dojindo) and incubated at 37 °C for at least 2 hours. Cell viability was determined by measuring the emission at 490 nm using 800TS Microplate Reader (BioTek).

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