NMR measurements on covalent adduct formation

DB Dániel Bálint
ÁP Ádám Levente Póti
AA Anita Alexa
PS Péter Sok
KA Krisztián Albert
LT Lili Torda
DF Dóra Földesi-Nagy
DC Dániel Csókás
GT Gábor Turczel
TI Tímea Imre
ES Eszter Szarka
FF Ferenc Fekete
IB Isabel Bento
MB Márton Bojtár
RP Roberta Palkó
PS Pál Szabó
KM Katalin Monostory
IP Imre Pápai
TS Tibor Soós
AR Attila Reményi
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NMR experiments were conducted on a Varian NMR System spectrometers operating at 600 MHz. Off-target adduct formation was analyzed through the reaction between 1aR-IN-8 and beta-mercaptoethanol (BME) by 1H NMR. All reagents were dissolved and the experiments were carried out in PBS (D2O, pH ~ 7.2) with 75% DMSO. Details of the measurements, the assignment of the spectra and Kchem determination are described in Supplementary Note 2.

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