NMR experiments were conducted on a Varian NMR System spectrometers operating at 600 MHz. Off-target adduct formation was analyzed through the reaction between 1aR-IN-8 and beta-mercaptoethanol (BME) by 1H NMR. All reagents were dissolved and the experiments were carried out in PBS (D2O, pH ~ 7.2) with 75% DMSO. Details of the measurements, the assignment of the spectra and Kchem determination are described in Supplementary Note 2.
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