Adaptation of adherent CHO cells to serum-free suspension culture

RH Ryan Haryadi
KC Kah Fai Chan
PL Pao Chun Lin
YT Yun Lei Tan
CW Corrine Wan
WS Wahyu Shahreel
ST Shi Jie Tay
TN Terry Nguyen-Khuong
IW Ian Walsh
ZS Zhiwei Song
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Adherent CHO cells were gradually transitioned to serum-free suspension culture by serially diluting the serum content in the growth medium over several passages with the appropriate suspension medium ratios. At 2.5% serum, the cell lines were transferred into shake flasks and cultured in incubator shaker until they were fully adapted to serum-free media.

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