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Hemoglobin Absorbance Measurement

GK George T Kannarkat
RZ Rebecca Zack
RS R Tyler Skrinak
JM James F Morley
RD Roseanne Davila-Rivera
SA Sanaz Arezoumandan
KD Katherine Dorfmann
KL Kelvin Luk
DW David A Wolk
DW Daniel Weintraub
TT Thomas F Tropea
EL Edward B Lee
SX Sharon X Xie
GC Ganesh Chandrasekaran
VL Virginia M-Y Lee
DI David Irwin
RA Rizwan S Akhtar
AC Alice S Chen-Plotkin
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Hemolysis was measured in plasma via absorbance to estimate the hemoglobin levels. Absorbance was measured at multiple wavelengths (380, 415, 450) and hemoglobin calculated in g/L based on this formula: (167.2 * A415 – 83.6 * A380 – 83.6 * A450) * 0.001 (62). Plasma samples with hemoglobin levels above 30 mg/dL were excluded from analyses.

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